A Chromeleon license file (.cmlic) is a small data file used by the Thermo Scientific Chromeleon Chromatography Data System (CDS) to authorize software use and enable specific purchased features. Core Content & Function
While the file is a binary/encoded data format rather than a standard text document you write yourself, it contains the following critical information:
Serial Number: A unique identifier (e.g., xxxxxx) that must match your physical USB license key (dongle).
Software Version: Specifies which version of Chromeleon (e.g., 7.2 or 7.3) the license is valid for.
Purchased Options: Activates specific software features such as: 3D data acquisition. Number of allowed client connections. Number of instrument controllers. Compliance features (Audit trails, etc.). Typical File Details Naming Convention: Chromeleon7_[SerialNumber].cmlic.
Standard Location: C:\ProgramData\Dionex\Chromeleon\ (This is a hidden folder by default). How to Obtain the File
You generally do not "write" this content. Instead, it is generated or transferred through these methods: chromeleon license file
From a Dongle: When you plug in a new light blue Chromeleon 7 USB dongle, the license file is often automatically transferred to your computer.
Online Activation: In newer versions (Chromeleon 7.3+), you can use dongle-free activation where you enter a License Initialization Code (LIC) to download the file directly from Thermo Fisher servers.
Technical Support: If you lose your file, you can contact Thermo Fisher support with your dongle's serial number to request a replacement .cmlic file. Licensing Codes (Management)
If you are managing licenses in the Administration Console, you will likely deal with these codes rather than the raw file content:
LIC (Initialization Code): Provided with your purchase to start the process.
LRC (Request Code): Generated by your PC to identify the installation. A Chromeleon license file (
LAC (Activation Code): The final code provided by Thermo Fisher to unlock the features.
Are you trying to recover a lost file or activate a new instrument for the first time?
Use a new Chromeleon 7.2 dongle that contains a license file
Knowing the default file paths is non-negotiable for troubleshooting. The location depends on your version and operating system.
For Chromeleon 7.x (Server-based installation):
C:\ProgramData\Thermo Scientific\Chromeleon\7\Data\C:\Program Files\Thermo Scientific\Chromeleon\For the License Manager Tool:
C:\Program Files (x86)\Thermo Scientific\Chromeleon\7\Tools\LicenseManager.exeImportant: The ProgramData folder is hidden by default in Windows. You must enable "View hidden items" in File Explorer to navigate there.
You cannot create a license file yourself. It must be generated by Thermo Fisher’s licensing portal based on your purchase order. Follow this standard workflow:
Chromeleon.lic file (usually as a .txt or .lic attachment). Do not open it in Notepad and resave it—this often corrupts the encoding.lmgrd + thermo.lic).Without a valid license file accessible to the server, Chromeleon will either fail to launch or run in a severely restricted “viewer-only” mode.
Old installations often stored licenses here:
C:\Chromeleon\Data\System\
C:\Program Files\Chromeleon\Shared\
Deploying the license file incorrectly is the #1 cause of "License Not Found" errors. Follow this protocol precisely:
Chromeleon often creates a backup. Look in:
C:\Program Files\Thermo\Chromeleon\License\backup\
or
C:\ProgramData\Thermo\Chromeleon\License\ For the License Manager Tool: